• Nicotiana Linnaeus, 1753
• Nicotiana acuminata (Graham) Hook.
• Nicotiana affinis T. Moore accepted as Nicotiana alata Link & Otto
• Nicotiana alata Link & Otto
• Nicotiana attenuata Torr. ex S. Wats.
• Nicotiana bigelovii (Torr.) S. Wats. accepted as Nicotiana quadrivalvis Pursh
• Nicotiana clevelandii Gray
• Nicotiana excelsior (J.M. Black) J.M. Black
• Nicotiana forgetiana Hort. ex Hemsley
• Nicotiana glauca Graham
• Nicotiana glutinosa Linnaeus
• Nicotiana langsdorffii Weinm.
• Nicotiana longiflora Cav.
• Nicotiana obtusifolia Mertens & Galeotti
• Nicotiana palmeri Gray accepted as Nicotiana obtusifolia Mertens & Galeotti
• Nicotiana paniculata Linnaeus
• Nicotiana plumbaginifolia Viviani
• Nicotiana quadrivalvis Pursh
• Nicotiana repanda Willd. ex Lehm.
• Nicotiana rustica Linnaeus
• Nicotiana sanderae hort. ex W. Wats.
• Nicotiana suaveolens Lehm.
• Nicotiana sylvestris Speg. & Comes
• Nicotiana tabacum Linnaeus
• Nicotiana tabacum Tnt1 virus
• Nicotiana tabacum Tto1 virus
• Nicotiana tomentosa Ruiz & Pavon
• Nicotiana trigonophylla Dunal accepted as Nicotiana obtusifolia Mertens & Galeotti